One conventional and two real-time PCR methods targeting a repetitive Sat-DNA sequence as well as one conventional PCR method targeting the variable region of the kDNA minicircle were compared for sensitivity, intra- and interassay precision, limit of detection, specificity and cross-reactivity. cruzi-positive and negative patients' blood samples and cultured T. This study compared four distinct PCR methods for detection of Trypanosoma cruzi DNA for the use in well-equipped routine diagnostic laboratories. Reliable diagnosis of chronically infected people is crucial for prevention of non-vectorial transmission as well as treatment. Seiringer, Peter Pritsch, Michael Flores-Chavez, MarÃa Marchisio, Edoardo Helfrich, Kerstin Mengele, Carolin Hohnerlein, Stefan Bretzel, Gisela Löscher, Thomas Hoelscher, Michael Berens-Riha, Nicoleĭue to increased migration, Chagas disease has become an international health problem. All rights reserved.Ĭomparison of four PCR methods for efficient detection of Trypanosoma cruzi in routine diagnostics. This assay could become useful in the rapid laboratory diagnostic assessment of the presence or absence of Naegleria fowleri. The reaction detection limit was 1 copy of the Mp2Cl5 DNA sequence. Using this method, no cross reactivity with other tested epidemiologically relevant prokaryotic and eukaryotic organisms was found.
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Here, we describe a real-time PCR-based diagnostic method using hybridization fluorescent labelled probes, with a LightCycler instrument and accompanying software (Roche), targeting the Naegleria fowleriMp2Cl5 gene sequence. While, traditional methods for diagnosing PAM still rely on culture, more current laboratory diagnoses exist based on conventional PCR methods however, only a few real-time PCR processes have been described as yet. Naegleria fowleri is a free-living amoeba that can cause primary amoebic meningoencephalitis (PAM). Madarová, Lucia Trnková, KatarÃna Feiková, Sona Klement, Cyril Obernauerová, Margita A real-time PCR diagnostic method for detection of Naegleria fowleri.
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